Sunday, 23 December 2018

Enzyme's unfrozen adventure: In crystallo protein thermodynamics

Researchers a have reported the first in crystallo thermodynamic analysis of copper amine oxidase catalysis using a non-cryocooled technique. The method, which analyzes protein crystals coated with a water-soluble polymer, rather than cryogenically cooled crystals, can be temperature-controlled, allowing for conformational changes to be monitored over a range of temperatures. Additionally, the conditions show parallels with the cytosol, meaning that the approach could provide a useful model for protein behavior in cells.

from Engineering and Construction News – ScienceDaily https://www.sciencedaily.com/releases/2018/12/181220104320.htm



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